This is the process I use to calibrate each filament. Part of the process I read about a while ago and for the life of me cannot find the site to give credit, if I do I will add it.

I start by printing the 20x20x5mm cube with the following settings in Simplify3d.

Extruder tab

• Extrusion Multiplier - 1.00
• extrusion width - manual - 0.48mm (assuming 0.40mm nozzle)

Layer Tab

• primary layer height - 0.20mm
• top solid layers - o
• bottom solid layers - 0
• outline/perimeter shells - 2

Infill - 0

Print the output stl and measure the width of the of the shells. the goal is 0.96mm if its under 0.96mm go back to simplify3d and adjust extrusion multiplier till you get as close as possoble to 0.96mm.

Once the extrusion multiplier is set turn to the 3 hole test stl.

I made this stl to test holes for a few things I use often, the holes are 6.5mm, 3.2mm and 2.7mm. I use the 6.5mm for SMA fitment, 3.2mm for M3 screw pass though and 2.7mm to thread an M3 screw into.

On the Other tab in Simplify 3d use the Horizontal size compensation to get the holes to the correct size. positive numbers make the holes smaller, negative numbers make the holes larger.