The shipping dewar positional cooling device (SDPCD, known as the ‘Cajun Ejector’) is a 3-D printable device that can assist cryopreservation with various standard cooling rates by use with shipping dewars. Efforts in development of germplasm repositories to preserve genetic resources of aquatic species are impeded globally by a lack of standardized, inexpensive, reproducible, and portable cryopreservation technologies.

The Micro Cajun Ejector a 3-D printed standardizable freezing device tailored specifically for the MVE BL-7 nitrogen vapor shipping dewar. This device holds 22 French straws (0.25-mL or 0.5-mL) and has a quick-release ring design to eject straws directly into the dewar by pressing a button after freezing. This device and the BL-7 are affordable, lightweight, and portable, making them ideal for field cryopreservation.

Instructions for assembling and usage are provided as user manuals separately. Please see the 'Other Files' tab to download the user manual.

The Fusion f3d files can be downloaded on the AGGRC GitHub page: https://github.com/aggrc/Micro-Cajun-Ejector

Details for the development and testing of the Original Cajun Ejector can be found in the publication: Childress, William M., Yue Liu, and Terrence R. Tiersch. "Design, alpha testing, and beta testing of a 3-D printed open-hardware portable cryopreservation device for aquatic species." Journal of Applied Aquaculture (2021): 1-24. DOI: 10.1080/10454438.2021.1955805

The compression spring can be purchased here:

https://www.amazon.com/gp/product/B008RG54YG/ref=ewc_pr_img_2?smid=ATVPDKIKX0DER&psc=1

Developed by the Aquatic Germplasm and Genetic Resources Center (AGGRC) at the Louisiana State University Agricultural Center. Designed by Brittany Ratliff and colleagues, in partnership with the International Zebrafish Resource Center (ZIRC) and USDA National Animal Germplasm Program (NAGP). This work was funded in part by NIH Office of Research Infrastructure Program (ORIP). This device is part of a multi-year project to develop a series of open hardware to support development of standardized and reproducible tools for the research communities that use aquatic models to study human diseases.